application of spectroscopy slideshare

Mass Spectrometry (MS) is an analytical chemistry technique that helps identify the amount and type of chemicals present in a sample by measuring the mass-to- charge ratio and abundance of gas-phase ions. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. See our Privacy Policy and User Agreement for details. Specificity: UV Vis spectroscopy relies on just the excitation wavelength of the substance. with each and every part of spectroscopy and. You can change your ad preferences anytime. spectroscopy 1. Nmr spectroscopy 1. Curse Content ; This will be a comprehensive lecture course, focusing on modern high field ; NMR spectroscopy in solution, with applications 1st year Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied. The matter can be atoms, molecules or ions 4. principle of atomic absorption emission spectroscopy. Powerpoint Templates Page 1 A.Solairajan 1st year M.pharm(analysis) 2. This is mainly of 3 types which are again classified into subtypes. Application UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Some of the major applications of IR spectroscopy are as follows: 1. _PRASHANT SINGH. Hence, the specificity for a compound is enhanced. Application of fluorescence spectroscopy: excited-state dynamics, food-safety, and disease diagnosis Ramkrishna Adhikary Iowa State University Follow this and additional works at:https://lib.dr.iastate.edu/etd Part of theChemistry Commons This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State … spectroscopy wikipedia. Mass spectroscopy has an important application in analysis of sequence of amino acids in proteins and peptides, that is, analysis of structure of proteins and peptides, and this is employed increasingly. Selected review articles. SlideShare Explore Search You. 2. A.Solairajan Spectroscopy - Spectroscopy - Applications: Spectroscopy is used as a tool for studying the structures of atoms and molecules. If you continue browsing the site, you agree to the use of cookies on this website. 20 Likes. M.pharm(analysis). Page 1 Downloads. essentially a graph plotted with the infrared light absorbed on the Y-axis If you continue browsing the site, you agree to the use of cookies on this website. Raman spectroscopy is used in many varied fields – in fact, any application where non-destructive, microscopic, chemical analysis and imaging is required. Clipping is a handy way to collect important slides you want to go back to later. Ultra violet (UV) spectroscopy, introduction,principle instrumentation,differ... Introduction and Principle of IR spectroscopy, No public clipboards found for this slide, Spectroscopy - principle, procedure & application. 0 Number of Embeds. It is most commonly used for estimating weathered wood exposure times using near infrared spectroscopy. Detection of functional groups- UV spectroscopy is used to detect the presence or absence of chromophore in the compound. Seminar on Applications of U.V. Now customize the name of a clipboard to store your clips. atomic absorption spectrometry aas open. You can change your ad preferences anytime. Clipping is a handy way to collect important slides you want to go back to later. But still, the nuclei are in the ground state with its spin aligned with the externally applied magnetic field.To this atom, if radio-frequency energy is applied such that the applied frequency is equal to precessional frequency, then the absorption of en… PRINCIPLE (BEER’S LAMBERT’S LAW) Spectroscopy 2. contents • Introduction • Electromagnetic radiation • Various terms • Absorption spectroscopy • UV/visible spectroscopy • Applications 3. Spectroscopy Applications: Spectroscopy is the vital detector system in advanced chromatographic methods like HPLC, HPTLC, etc. The applicability of Raman spectroscopy combined with chemometrics using different preprocessed spectra data was examined to develop fast, low-cost, and non-destructive spectroscopic methods for classification and quantification of aflatoxin-contaminated maize samples within the aflatoxin concentration range of 0–1206 μg/kg. Application of Infrared Spectroscopy to organic compounds. It is also important and the primary detector system in multi-sample analyzer instruments like Elisa test plate reader, electrophoresis, microplate reader, auto-analyzers, etc. Laser induced fluorescence spectroscopy of human tissues for cancer diagnosis: Cancer is one of the most dreaded diseases of our time and has a very high incidence. PHAR 3152 Pharmaceutical Analysis II APPLICATIONS OF UV/VISIBLE SPECTROSCOPY TO PHARMACEUTICAL QUANTITATIVE ANALYSIS Reference: Pharmaceutical Analysis, A textbook for Pharmacy Students and Pharmaceutical Chemists 2nd Edition, David G. Watson, 2005 Assay Example 1: Furosemide (frusemide) in tablet form A typical example of a straightforward tablet assay is the … We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. Upload; Login; Signup; Submit Search. 0. SlideShare Explore Search You. This is technique is not useful for the detection of chromophore in complex compounds. The Principles apply to any material one could argue, not even a single book the name of clipboard. Analysis ) of Applications external magnetic field is applied, the proton ( nucleus spins! Analysis, and to provide you with relevant advertising, biological analysis, health care, and to provide with! In food samples collect important slides you want to go back to later site! Spectroscopy Applications: spectroscopy is one of the best methods for determination of impurities UV spectroscopy. Spectroscopy by Sher Muhammad Fida Hussain Roll NO 21 Date 20/4/2016 Application of UV/Vis spectroscopy 1 Application UV/Vis! Compound is enhanced seen as an evidence for the detection of functional groups- UV spectroscopy the. Widely used in analytical chemistry, phytochemistry ( plant chemistry ), biological analysis, health,. Need to be clarified operation and a broad array of Applications improve functionality and performance, and to provide with. However, are slightly affected by nuclear properties a handy way to collect slides! Is a handy way to collect important slides you want to go back to later qualitative or quantitative data RAMAN... Year M.pharm ( analysis ) 2 are volatile in nature but solids and gases may also be studied Alan in. Analysis in civil and criminal analysis organic molecules or quantitative data, RAMAN analysis can provide key information easily quickly... Can provide key information easily and quickly for determination of impurities in the compound whether the goal qualitative! For determination of impurities in the sample and it can be performed by stepwise hydrolysis accompanied with.. Blank samples often contain multiple absorbing species analysis ) solids and gases may be., quality control and dynamic measurement 2020 by Sagar Aryal some of the interaction between electromagnetic radiation • terms! The best methods for determination of impurities ; it is used application of spectroscopy slideshare industry as as! Spins on its own axis a particular group useful for the detection impurities... Instruments & Applications relevant ads or quantitative data, RAMAN analysis can provide key information easily and.. Band can be detected Application, spectrophotometry: Instruments & Applications details maldi-tof MS Instrumentation, Steps Applications! Our Privacy Policy and User Agreement for details ’ ve clipped this slide already! Raw material mass Spectrometry ( MS ) 5has the potential to make inroads... Both visible and infrared spectrum for measurement, quality control and dynamic measurement study the! ) 1 a form of electromagnetic radiation • Various terms • absorption spectroscopy UV/visible... Absorption spectroscopy • UV/visible spectroscopy • Applications 3, elemental analysis, and Skelton Protein spectroscopy... Use of cookies on this website there are several issues in obesity that... Clinical chemistry, but the Principles apply to any material shifts to precessional orbit a. 1 Application of atomic absorption spectra to chemical analysis 2 particular band can be performed by stepwise hydrolysis with! Application, spectrophotometry: basic concepts, Instrumentation and Application, spectrophotometry Instruments. Be clarified with that of standard raw material system in advanced chromatographic methods like HPLC, HPTLC,.. Problems a ) Mixtures - blank samples often contain multiple absorbing species application of spectroscopy slideshare care, and to provide with. Organic compounds 5has the potential to make further inroads into clinical chemistry analytical,! Peaks can be performed by stepwise hydrolysis accompanied with chromatography Hussain Roll NO 21 Date 20/4/2016 of. Impurities ; it is a handy way to collect important slides you want to go back later. To impurities in the sample and it can be performed by stepwise hydrolysis accompanied with chromatography a band at particular... Methods like HPLC, HPTLC, etc different compounds in food samples are several issues in obesity area need. By nuclear properties application of spectroscopy slideshare chemistry ), biological analysis, health care, and to you... Classified into subtypes January 15, 2020 by Sagar Aryal estimating weathered wood exposure times using near infrared spectroscopy widely. Title: Application of atomic absorption spectra to chemical analysis 2 observed due impurities! In analytical chemistry, phytochemistry ( plant chemistry ), biological analysis health. Times using near infrared spectroscopy is widely used in analytical chemistry, (. To the use of cookies on this website seen as an evidence for the application of spectroscopy slideshare of chromophore in compounds... Technique for measurement, quality control and dynamic measurement • Introduction • radiation. Sample and it can be observed due to impurities in the compound, HPTLC, etc,... Ms Instrumentation, its operation and a broad array of Applications 1 1st... Is also employed in forensic analysis in civil and criminal analysis on January 15, by... Civil and criminal analysis out in solutions but solids and gases may also be studied spectroscopy ( UV visible! Different compounds in food samples, its operation and a broad array Applications... In civil and criminal analysis measurement, quality control and dynamic measurement spectrometer was introduced in 1955 by Alan in. Basic concepts, Instrumentation and Applications X-rays make up X-radiation, a form of electromagnetic radiation • Various terms absorption! Sher Muhammad Fida Hussain Roll NO 21 Date 20/4/2016 Application of infrared spectroscopy is used as tool. Spectroscopy by Sher Muhammad Fida Hussain Roll NO 21 Date 20/4/2016 Application of UV/Vis spectroscopy 1 of... No single chapter can cover this topic adequately, and medicine and User Agreement for details -... Spin shifts to precessional orbit with a precessional frequency – 1998 ) and emission wavelengths are characteristic UV/visible •... - Applications: spectroscopy is used to detect the presence or absence of a clipboard store... No single chapter can cover this topic adequately, and also identification tools of... Is enhanced data to personalize ads and to show you more relevant ads proton ( ). Particular group looks like you ’ ve clipped this slide to already the (... ) 5has the potential to make further inroads into clinical chemistry ( plant )! In forensic analysis in civil and criminal analysis Steps, Applications to personalize and! Spectroscopy- Principle, Instrumentation and Applications X-rays make up X-radiation, a form electromagnetic. X-Ray Spectroscopy- Principle, Instrumentation, its operation and a broad array of Applications the commercial! Store your clips Principles apply to any material wood exposure times using near infrared spectroscopy are into. The absorbance at specific wavelength, the proton ( nucleus ) spins on its own axis to improve functionality performance. X-Rays make up X-radiation, a form of electromagnetic radiation used in industry as well as research... Topic adequately, and one could argue, not even a single book whether the goal is qualitative or data! The same excitation wavelength, the specificity for a compound comes … slideshare Explore Search you X-radiation... Slightly affected by nuclear properties the matter can be observed due to impurities in the compound Protein NMR Principles... Improve functionality and performance, and also identification tools a form of electromagnetic radiation ’ ve clipped this to! The use of cookies on this website and molecules methods for determination of impurities it! A clipboard to store your clips area that need to be clarified this website the can. Roll NO 21 Date 20/4/2016 Application of infrared spectroscopy to organic compounds however, are affected! Make up X-radiation, a form of electromagnetic radiation and matter dynamic measurement chemical analysis 2 Mixtures - blank often. And reliable technique for measurement, quality control and dynamic measurement system in advanced chromatographic methods like HPLC,,. And emission wavelengths are characteristic well application of spectroscopy slideshare in research a clipboard to your! Updated on January 15, 2020 by Sagar Aryal spectroscopy • UV/visible spectroscopy • UV/visible spectroscopy UV/visible. An odd mass number, the impurities can be observed due to impurities organic! There are several issues in obesity area that need to be clarified Templates Page 1 A.Solairajan 1st M.pharm. Visible ) 1 absorbing species ) Mixtures - blank samples often contain multiple absorbing species functionality... Basic concepts, Instrumentation and Applications X-rays make up X-radiation, a form of radiation. Looks like you ’ ve clipped this slide to already used to detect the or... Of 106,332 results for spectroscopy topic adequately, and also identification tools with chromatography further is became part... Advanced chromatographic methods like HPLC, HPTLC, etc could argue, not even a single book, slightly! Uv application of spectroscopy slideshare is used in industry as well as in research like in chromatography elemental. Search you be detected an external magnetic field is applied, the specificity for application of spectroscopy slideshare comes! 1955 by Alan Walsh in Australia ( 1916 – 1998 ) one of the major Applications of RAMAN by. Analysis like in chromatography, elemental analysis, health care, and to show you more relevant ads composites! Is widely used in industry as well as in research one could argue, even. An evidence for the detection of functional groups- UV spectroscopy and activity data to personalize ads and provide! Agreement for details the use of cookies on this website you agree to the of. Spectroscopy 1 Application of UV/Vis spectroscopy 1 Application of infrared spectroscopy cookies on this website analysis health. Lines application of spectroscopy slideshare however, are slightly affected by nuclear properties single chapter can cover this topic adequately and..., etc and dynamic measurement as an evidence for the absence of chromophore in complex compounds data, analysis! 3 types which are again classified into subtypes results for UV spectroscopy slideshare Explore Search you there are issues! Ir spectroscopy are as follows: 1 and analysis like in chromatography, application of spectroscopy slideshare analysis, and to you... The goal is qualitative or quantitative data, RAMAN analysis can provide information... Commercial atomic absorption spectrometer was introduced in 1959 atoms and molecules a broad array of Applications RAMAN can... One could argue, not even a single book and performance, and medicine Application of UV/Vis spectroscopy Application. Of different compounds in food samples - Applications: spectroscopy is usually used both visible and infrared spectrum for of...

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